Hemoglobin Brockton [beta 138 (H16) Ala—-Pro] has reduced oxygen affinity but electrophoretic mobility and hemolysate binding are regular, suggesting the substitution at beta 138 doesn't disrupt significant polar interactions, including the hydrogen bonds and salt bridges concerning Asp 137 and Pro 138, that lead on the cooperative oxygen-binding course of action in Hb A. The variant is unstable and has become linked to mild anemia.
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